What is the difference between hplc and uplc

The different types of chromatography are paper chromatography, thin-layer chromatography TLC , column chromatography, partition chromatography, high-performance liquid chromatography, and gas chromatography are commonly used for qualitative as well as quantitative determination of molecules.

What is HPLC? Both high-performance liquid chromatography and ultra-performance liquid chromatography are the separation techniques used for separate different components found in the complex mixture.

There we can determine the differences in retention times for each component in the sample. It is a variation of HPLC, but the method of operation is the same. However, unlike HPLC, this method allows very small particles to be analyzed, such as 2 micrometres small particles. Moreover, it provides a faster analysis. In this technique, we use a MPa pump pressure for the insertion of the sample into the column. Hence, this high pressure confirms that the whole sample is introduced into the column.

Thus, this is very efficient compared to HPLC. Thus, the method of operation is the same for both techniques. Less back pressure, more life of column 6. Has more use 11 Higher precision in sample introduction is required Lower comparative to UPLC 12 Detectors uses small flow rates and low detection volume, Hence high data sampling rate is required. Detectors uses higher flow rates and high detector volume 7.

Inorganic Silica modified to different polarity is generally used. UPLC HPLC 16 More plate count is achieved during analysis Less plate count is achieved during analysis 17 System should withstand high pressures, more efficient pumps are required Less efficient pumps are required 18 Low extra column dispersion More extra column band broadening.

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